CIAO1 and malignant colon neoplasm: Further analysis of RNA-seq data for CIAO1, (HEK293, 1CT, and SW620 colon cancer cells) revealed a relatively high number of reads (comparable to read numbers mapping to the flanking exons and the 3′ UTR) present in the region that corresponds to the CIAO1 penultimate intron (Fig. 4B).