PTCH1 and nevoid basal cell carcinoma syndrome: Because mutations, or single nucleotide variations (SNVs), as well as CNAs of PTCH1 and other genes related to the Hedgehog signaling pathway-related genes with numerous exons can be responsible for Gorlin syndrome, we conducted hybridization and polymerase chain reaction (PCR)-based next generation sequencing (NGS) analyses in a cohort of Gorlin syndrome-affected individuals from six unrelated families [13, 14] for a combined screening of causative alterations in candidate genes.