One potential marker (extracellular matrix protein 1 (ECM1) previously identified and associated with BC), and two novel potential protein markers (MAST4-microtubule associated serine/threonine kinase family member 4 and filaggrin) identified from BC urine were validated in BC cell lines and MAST4 was validated in a small number of primary BC tissues and in the individual human BC urine samples, demonstrating the link of these proteins with BC. The gene discussed is ECM1; the disease is breast cancer.