We reported here that in bladder cancer cells, KLF5-VEGFA axis was activated by growth factor EGF and bFGF, PKC agonist PMA and mitogenic lipid LPA, but inhibited by inhibitors of EGFR (AG1478), MEK1/2 (U0126) or PI3K (LY294002). Here, KLF5 is linked to urinary bladder cancer.