To investigate whether Cullin3 is involved in the BRMS1 degradation in BC cells, the half-life period of BRMS1 in MDA-MB-468-Cullin3 and its control cells were measured by Western blot, and we found that when ectopic Cullin3 in MDA-MB-468 cells the half-life period of BRMS1 was significantly decreased (Figure 8E). The gene discussed is BRMS1; the disease is breast cancer.