As shown in Figure 6, immunostaining of Cav-1 in tissue sections obtained from healthy mammary glands of FVB controls as compared to mammary tumors from ErbB2 mice showed generalized reduction of Cav-1 expression in all ErbB2 mouse tumors analyzed, which also displayed elevated MnSOD expression and increased AMPK (Thr172) phosphorylation. This evidence concerns the gene SOD2 and breast cancer.