We examined the relative gene expression levels in the amiRNAs-transfected bladder cancer cell lines and the mRNA levels of c-Myc (Fig. 2A,B), cyclin D1 (Fig. 2A,B), IGF2 (Fig. 2C,D), and VEGF (Fig. 2C,D) were down-regulated by the corresponding amiRNA in the bladder cell lines. This evidence concerns the gene CCND1 and urinary bladder cancer.