CHGA and pachyonychia congenita: To assess whether SNAI2's ability to boost NED genes may involve SOX2, we transfected DU145 cells (which have low levels of endogenous SNAI2 mRNA with respect to other PC cell lines and low constitutive level of SOX2 mRNA, as shown in ref. 14) to overexpress SNAI2 and silence SOX2. SOX2 silencing (by 75%) in DU145 cells resulted in a significant (p < 0.05) downmodulation of both CHGA (by 76%) and SYP (by 63%).