To identify if IGFBP3 is required for tumor growth and lymphatic metastasis, we generated 3 LN1–1 subclones that stably express 2 different IGFBP3 shRNA and one control vector (pLKO-GFP) to access the effects of IGFBP3 silencing in LN1–1 cells. This evidence concerns the gene IGFBP3 and neoplasm.