FAS and Hepatic steatosis: The lipoogenic genes including fatty acid synthase (FAS), acetyl-coenzyme A carboxylase 1 (ACC1), and diacylglycerol acyltransferase (DGAT) decreased in tunicamycin-treated HepG2 cells and mice even though the mice had hepatic steatosis (data not shown), which was consistent with previous reports [16,17].