UCHL1 and infection: To explore which cysteine residues in UCH-L1 are the primary targets for 15dPGJ2 adduction, Flag-tagged UCH-L1 was overexpressed in the rat primary neurons by lentivirus (LV)-Flag UCHL-WT infection and cells were then incubated with 15dPGJ2 (20 μM) for 24 h before harvest.