Immunoblot analysis showed that CD133 was highly expressed in NTERA2 and NCCIT cells (Figure 1c and Supplementary Figure 1), which are derived from poorly differentiated germ cell tumors and utilized as CSC model cell lines.28 Although the p53 expression levels differed greatly among the various cancer cell lines, p53 expression was inversely correlated with CD133 expression. The gene discussed is PROM1; the disease is testicular germ cell tumor.