In this context, we have previously demonstrated that E-cadherin from human colon carcinoma HCT-116 cells, which have unstable AJs, is more richly N-glycosylated than E-cadherin from Caco-2 cells, which have stable AJs, in addition, the inhibition of N-glycan biosynthesis induces a functional E-cadherin-mediated cell-cell adhesion in HCT-116, which was disrupted by calcium depletion [37]. The gene discussed is CDH1; the disease is colon carcinoma.