Cancerous tissues spread downwards to the stroma outside the cervical glands; however, because cervical tissue itself is much thinner than the myometrium, malignant tissue infiltration depth at stage II endometrial cancer may be less than that at stage I. Theoretically, the expression level of HE4 may be lower than that at stage I, which is in accordance with our study: with the exception of stage II, the positive expression and strongly positive expression rate of HE4 increased with increasing stage. The gene discussed is WFDC2; the disease is endometrial cancer.