HE4 positive expression rate in patients with lymph node metastasis was 89.66%, close to 84.93% in patients without lymph node metastasis (P = 0.532), whereas the strongly positive expression rate of HE4 in patients with lymph node metastasis (73.33%) was significantly higher than that in patients without lymph node metastasis (41.40%) (P = 0.002). This evidence concerns the gene WFDC2 and metastatic malignant neoplasm in the lymph nodes.