To examine the effects of A20 on the proliferation of HCC cells, we established A20 overexpression or knockdown stable HCCLM3 cells (LM3-A20 and LM3-shA20, respectively) and their empty vector counterparts (LM3-con and LM3-shcon, respectively) (Fig. 3a). The gene discussed is TNFAIP3; the disease is hepatocellular carcinoma.