Annexin-V-FITC/PI double staining assay and FCM analyses showed that apoptotic cells in the untreated human breast cancer cells MCF-7 and MDA-MB-231 were 4.70.11 ± 1.41 and 3.90 ± 1.27 %, respectively, after treatment with 4, 6, 8 μg/ml of morusin for 48 h, the apoptotic cells in the treated cancer cells increased significantly (p < 0.01, Fig. 3), in a dose-dependent manner. This evidence concerns the gene ANXA5 and breast carcinoma.