HSF1 and lentivirus infection: To examine the latency-reversing efficiency of the identified msgRNAs LTR-L and O in TZM-bI HIV-1 latent cells, we established a dCas9-VPH-expressing stable cell line by lentivirus infection and double selection with puromycin for dCas9-VP64 and hygromycin for MS2-p65-HSF1 followed by lentiviral transduction with indicated msgRNAs.