However, by quantifying MS images, we first reported that quantitative results of HER2 expression had relation to the lymph node status and histological grades of invasive BC; the total signal OD values were higher in lymph-node-positive than in lymph-node-negative tumors and in high-grade tumors than in middle-grade tumors (P = 0.02 and 0.04). This evidence concerns the gene ERBB2 and breast cancer.