Immunohistochemical (IHC) assays of specimen from internal and external validation cohort (Supplementary Figure S1 and Supplementary Table S1) showed Ack1 expression was highly located in the cytoplasm (Figure 1Ca), and further validated that the expression level of Ack1 was significantly higher in HCC tumors than that in ANLTs (Figure 1Cb & 1Cc). Here, TNK2 is linked to hepatocellular carcinoma.