To study the biological significance of Ack1 in HCC invasion and metastasis, the expression levels of Ack1 in normal liver cell lines L02 and four HCC cell lines with various metastatic capability, including HepG2, MHCC97-L and HCCLM3, Huh7, were measured (Figure 3A). Here, TNK2 is linked to hepatocellular carcinoma.