Ack1 overexpression could induce the reorganization of actin cytoskeleton and modulate HCC cells into a mesenchymal-like morphology (Supplementary Figure S3C), while down-regulation of Ack1 changed the cell appearance to more cobble-stone morphology (Supplementary Figure S3D), indicating that Ack1 was involved in a cellular transition termed epithelial-mesenchymal transition (EMT). This evidence concerns the gene TNK2 and hepatocellular carcinoma.