To determine Ack1 function in HCC invasion and metastasis, we overexpressed Ack1 in MHCC97-L (named MHCC97-LAck1, Supplementary Figure S3A) and knocked down Ack1 in HCCLM3 cells (named HCCLM3shAck1, Supplementary Figure S3B). The gene discussed is TNK2; the disease is hepatocellular carcinoma.