To further characterize the functional significance of p-NPM-Thr234/237 in HCC, we first subcloned Flag-tagged NPM and its phosphorylation site mutant (Thr234/237A) into the pCDH vector (System Biosciences) and overexpressed the gene in low p-NPM-Thr234/237 expressing cells (Hep3B and Huh7) using a lentiviral transduction system (System Biosciences). The gene discussed is NPM1; the disease is hepatocellular carcinoma.