Using CelluSpotTM Serine/Threonine kinase peptide array analysis, we compared the phosphorylation profiling of the two matched HCC cell lines and found phosphorylated level of nucleophosmin (NPM) at Threonine 234/237 (p-NPM-Thr234/237) had remarkably high level in metastatic HCC cells (PLC-LM) than the corresponding primary HCC cell line (PLC-PT). Here, NPM1 is linked to hepatocellular carcinoma.