Broadly, all peptides were capable of stimulating T-cells from both healthy donors (n = 4) and patients (n = 4), where the mean cytokine-secreting CTLs was significantly higher in T-cells from NPC patients compared to healthy donors for both IFN-γ (healthy donors: NPC patients = 80.0 ± 106.7: 307.3 ± 276.5; p = 0.003) and granzyme B (healthy donors: NPC patients = 71.0 ± 77.8: 280.3 ± 217.0; p < 0.001) (Fig 4a and 4b). Here, GZMB is linked to nasopharyngeal carcinoma.