To deplete the extracellular asparagine, PbASKO-infected mice were injected with three doses of L-asparaginase (50 IU per mouse), conforming to the ALL treatment regimens in children and adults that involve multiple doses of L-asparaginase (E. coli, Erwinia or pegylated)22, 23 administered over several days (5,000–10,000 IU m−2 for E. coli asparaginase)22, 23, 45. The gene discussed is ASPG; the disease is acute lymphoblastic leukemia.