Next, we confirmed these findings by qRT–PCR for the TNF-α responsive genes IL1B and IL6 using independent MITF siRNAs in MZ7 and two other MITFhigh melanoma cell lines, Ma-Mel-15 (MM15) and Ma-Mel-27 (MM27), respectively (Fig. 2f and Supplementary Fig. 2). This evidence concerns the gene IL1B and melanoma.