EGF and lung cancer: Notably, monitoring phosphorylation of Akt at T308 has been shown to improve the assessment of Akt activation and revealed that Akt activation is a poor prognostic factor for lung cancer [22], we thus examined the activation of AKT in association with miR-622 expression level during hypoxia; however, T308 of AKT kinase was not phosphorylated in response to EGF treatment in A549 cells (Figure 5B).