HIF1A and lung cancer: In parallel, results from HIF-1α silencing experiments with two short hairpin RNAs (shRNAs) targeting HIF-1α mRNA (shHIF-1α) revealed dramatic decreases in the migration and invasion of lung cancer cells (Figures 3G–3I), lending support to our theory that miR-622 inhibits tumor motility via repression of HIF-1α to down-modulate the EMT axis.