At 7 days after DOX-induction of the CTNNB1 shRNAs, in the three colon cancer cell lines, we found moderate (HCT116) to dramatic (DLD1 and SW480) decreases in the active pool of β-catenin protein with only modest changes in total β-catenin protein levels (S8 Fig). Here, CTNNB1 is linked to malignant colon neoplasm.