GLS and neuroblastoma: Using an antibody recognizing both isoforms of GLS1, we only detected the 53 KD protein band of GAC isoform in neuroblastoma cell lysates (Figure 1D), which was further confirmed by shRNA depletion in additional neuroblastoma cell lines (Supplementary Figure S1), demonstrating that GAC is the predominant GLS1 isoform expressed in human neuroblastoma cells.