Interestingly, all melanoma cell lines tested (SKMEL28, 501MEL, A375P, and WM266.4) increased their roundness index and their levels of actomyosin contractility (measured as MLC2 phosphorylation) after treatment with TGF-β (Figures 1A–1C; Figure S1A). Here, TGFB1 is linked to melanoma.