NTS and persistent truncus arteriosus: In addition, treatment of rat neonatal cardiomyocytes with TAC-operated mouse serum induced significantly higher deacetylase activity of Sirt1 than that with sham-operated mouse serum (Fig. 3c), suggesting that increase in eNampt-dependent biosynthesis of plasma NMN was sufficient for activation of Sirt1 in myocardium.