Since the levels of IL-4 and IL-10 remained decreased despite the chronic imipramine treatment, we supplemented 100 ng of each mouse recombinant IL-4 and IL-10 to co-Imi+CRS mice to assess whether immune balance by these supplements can prevent the induction of depression-like behaviors upon an exposure to an additional stressful stimulus. Here, IL10 is linked to major depressive disorder.