First, we transfected Neuro2A cells with pCMV-hUBQLN2WT or pCMV-hUBQLN2P497H to determine if inclusions were formed and if they exhibited ALS-like features with aggregated TDP-43 or p62 proteins and if positive for NF-κB associated proteins. Here, SQSTM1 is linked to amyotrophic lateral sclerosis.