Although we did not find a significant correlation between tumor size and TILs (CD3: r = 0.031, p = 0.7968; CD8: r = 0.0223, p = 0.8648) in melanomas, still the PD-1/CD8 ratio was strongly negatively correlated with brain metastases size (r = 0.3331, p = 0.0093) (data not shown). This evidence concerns the gene CD8A and melanoma.