To investigate whether PVT1 suppresses apoptosis of bladder cancer cells, cells were transfected with si-PVT1 or si-NC and cell apoptosis was detected by the caspase 3 enzyme-linked immunosorbent assay (ELISA), Hoechst 33258 staining assay and flow cytometry assay. Here, CASP3 is linked to urinary bladder cancer.