PVT1 and urinary bladder carcinoma: CCK8 asasy suggests that compared with the negative control, tetracycline-inducible PVT1 shRNA reduced cell proliferation significantly when added with 1 ug/ml doxycycline in bladder cancer T24 and 5637 cells (P < 0.001 in two cell lines) (Figure 3C and 3D).