EML4 and non-small cell lung carcinoma: To demonstrate whether EML4-ALK directly enhances the stem-like phenotypes of NSCLC cells, we retrovirally-transduced EML4-ALK− A549 NSCLC cells with EML4-ALK variant 1 (A549/EAV1) or empty vector (A549/no insert), and then characterized their stem-like phenotypes (Figure 2).