Our results demonstrated the inhibitory effect of DSF on ERK/NF-κB/Snail pathway, and direct role of inhibition of ERK/NF-κB/Snail on the regulation of EMT and CSCs in breast cancer was tested by U0126, a chemical inhibitor of MEK1/2 - the upstream activator of ERK. This evidence concerns the gene SNAI1 and breast carcinoma.