TWIST1 and neuroblastoma: In order to further confirm Wnt activation, we analysed induction of several established target genes of canonical Wnt signalling in the NB cell lines treated with Wnt3a/Rspo2, including AXIN2, BMP4, JAG1, TNFRSF19, and TWIST1 (http://web.stanford.edu/group/nusselab/cgi-bin/wnt/).