Results showed that a trend of methylation was associated with higher expression of the target genes, as indicated by the lower ΔCt (Ct MADD/PIK3R2 - Ct GAPDH) in methylated cell lines (MM or MU), as compared with those in unmethylated cell lines (UU) (Supplementary Figure S3), confirming the role of methylation of miR-3151 in the regulation of MADD and PIK3R2 in CLL cells. This evidence concerns the gene PIK3R2 and B-cell chronic lymphocytic leukemia.