NTCP expression in the indicated HCC cell lines and primary hepatocytes has also been detected by Yan et al in lab experiment, the results showed that the levels of NTCP mRNA in Huh-7 and HepG2 cells were about 10,000 times lower than that in primary human and Tupaia hepatocytes, which was consistent with our observation in clinical tissues [19]. This evidence concerns the gene SLC10A1 and hepatocellular carcinoma.