The results showed that high SPC24 expression (p < 0.001), size of tumor >5 cm (p < 0.001), multiple tumor number (p < 0.001), B-C of BCLC stage (p < 0.001), the presence of PVTT (p < 0.001), and distant metastasis (p = 0.034) were significantly associated with poor DFS rate in HCC patients. Here, SPC24 is linked to hepatocellular carcinoma.