To directly investigate the differentiation potential of cardiac c-kit+ cells post myocardial infarction, we ligated the left anterior descending (LAD) coronary artery of c-kitH2B-tdTomato/+;Nkx2.5H2B-GFP/+ mice (2–5 months old, n=12, Fig. 5a,b). This evidence concerns the gene KIT and myocardial infarction.