However, there was no significantly difference among CUDR knocked down, CUDR knocked down plus CyclinD1 overexpressed, CUDR knocked-down plus PTEN knocked-down non-HLCSC and control liver cancer unstem cells (the colony-formation was 29.85 ± 7.82%, 32.46 ± 8.14%, 33.18 ± 6.39%, 31.45 ± 6.31%, P > 0.05). This evidence concerns the gene PTEN and liver cancer.