Moreover, there is also no significant difference among liver cancer unstem cells groups transfected with pCMV6-A-GFP, pCMV6-A-GFP-CUDR, pCMV6-A- GFP-CUDR plus pGFP-V-RS-CyclinD1, pCMV6-A-GFP-CUDR plus pcDNA3.1-PTEN respectively (P > 0.05). The gene discussed is PTEN; the disease is liver cancer.