To address whether CUDR overexpression cooperated with cyclinD1 overexpression or PTEN knockdown to accerlate the liver cancer stem cell proliferation, we established the stable human liver cancer stem cell(HLCSC) lines transfected with pCMV6-A-GFP, pCMV6-A-GFP-CUDR, pCMV6-A-GFP-CUDR plus pcDNA3.1-CyclinD1, pCMV6-A-GFP-CUDR plus pGFP-V-RS-PTEN, respectively. The gene discussed is CCND1; the disease is liver cancer.