To address whether CUDR knockdown cooperated with cyclinD1 overexpression or PTEN knockdown to influence on the liver cancer stem cell proliferation, we established the stable human liver cancer stem cell(HLCSC) lines transfected with pGFP-V-RS, pGFP-V-RS-CUDR, pGFP-V-RS-CUDR plus pcDNA3.1-CyclinD1, pGFP-V-RS-CUDR plus pGFP-V-RS-PTEN, respectively. The gene discussed is PTEN; the disease is liver cancer.