To address whether CUDR overexpression cooperated with cyclinD1 knockdown or PTEN overexpression to influence on the liver cancer stem cell proliferation, we established the stable human liver cancer stem cell(HLCSC) lines transfected with pCMV6-A-GFP, pCMV6-A-GFP-CUDR, pCMV6-A-GFP-CUDR plus pGFP-V-RS—CyclinD1, pCMV6-A- GFP-CUDR plus pcDNA3.1-PTEN, respectively. This evidence concerns the gene PTEN and liver cancer.