To address whether CUDR combined cyclinD1 or PTEN knockdown collectively alters C-myc expression, we first performed the Co-Immunoprecipitation(IP) in stable liver cancer stem cells transfected with pCMV6-A-GFP, pCMV6-A- GFP-CUDR, pCMV6-A-GFP-CUDR plus pcDNA3.1-CyclinD1, pCMV6-A- GFP-CUDR plus pGFP-V-RS PTEN, respectively. Here, PTEN is linked to liver cancer.