However, there was no significantly difference among CUDR overexpressed, CUDR overexpressed plus CyclinD1 overexpressed, CUDR overexpressed plus PTEN knocked-down and control liver cancer unstem cells (the colony-formation was 31.54 ± 6.12%, 35.34 ± 4.78%, 30.21 ± 7.81%, 28.76 ± 5.23%, P > 0.05, respectively). The gene discussed is CCND1; the disease is liver cancer.