Moreover, there was no significantly difference among CUDR overexpressed, CUDR overexpressed plus CyclinD1 knocked-down, CUDR overexpressed plus PTEN overexpressed and control liver cancer unstem cells (the colony-formation was 31.74 ± 6.78%, 26.89 ± 4.67%, 30.45 ± 7.12%, 27.45 ± 5.67%, P > 0.05). This evidence concerns the gene CCND1 and liver cancer.