The only phenotypes affected by GFP-Nek2 expression in shE2F3 cells -detected by immunofluorescence of tumor sections- were increased CA and proliferation (Figure 2C), lower levels of E-Cadherin detected by western blots (Figure 2D and 2E) and the induction of invasive protrusions (Figure 3), suggestive of increased detachment of cells from acinar structures, one of the initial steps in the invasion process. This evidence concerns the gene CDH1 and neoplasm.