To clarify whether the levels of miR-214 and miR-126 were increased in the plasma of canines with HSA, we examined the levels of miR-214, miR-126, and miR-16 in the plasma of canines with HSA (HSA group, n = 10), benign splenomegaly (benign group, n = 9), and in that of control canines with no specific disease (control group, n = 10) by performing miRNA qRT-PCR. The gene discussed is ALB; the disease is Splenomegaly.