Assessment of the influence of glutamine on NAFLD induced in rats by a high fat diet (HFD) revealed that hepatic steatosis was accompanied by significant increased liver lipid peroxidation, tumor necrosis factor α (TNF-α) levels, and of p65 NF-κB expression, with concomitant glutathione (GSH) depletion. This evidence concerns the gene TNF and metabolic dysfunction-associated steatotic liver disease.