In model II, serum bilirubin levels >1.6 g/dL (HR 1.540, 95% CI: 1.080–2.196, and P = 0.017), platelet ≤105/mm3 (HR 1.362, 95% CI: 1.012–1.833, and P = 0.042), AFP >20 ng/mL (HR 1.949, 95% CI: 1.491–2.549, and P < 0.001), tumor size >3 cm (HR 1.508, 95% CI: 1.143–1.989, and P = 0.004), and treatment modality (RFA, HR 1.709, 95% CI: 1.223–2.389, and P = 0.002; TACE, HR 2.391, 95% CI: 1.619–3.530, P < 0.001; and others, HR 4.138, 95% CI: 2.329–7.352, and P < 0.001) were the independent risk factors associated with poor overall survival. The gene discussed is AFP; the disease is neoplasm.