Multivariate analysis showed that AFP >20 ng/mL (P < 0.001), tumor size >3 cm (P = 0.022), and treatment modalities (resection as reference, RFA, P = 0.012; TACE, P = 0.001; and other therapies, P < 0.001) were the independent poor prognostic factors in these patients (Table 4, Fig. 2E). The gene discussed is AFP; the disease is neoplasm.