Downregulation of HIC1 was detected in all six ESCC cell lines (KYSE180, KYSE410, KYSE1170, EC1, EC18 and EC109), while the normal expression of HIC1 was detected in human normal esophageal epithelial cell line HEEC (Figure 1A and 1B). This evidence concerns the gene HIC1 and esophageal squamous cell carcinoma.