Next we used ENCODE data to analyze transcriptome profiles available for HMEC, many other normal cell cultures and MCF-7 in the ENCODE database (ENCODE/RNA-seq/Cold Spring Harbor Lab) to elucidate the positive association shown in Table 5 between cancer DNA hypermethylation and gene expression for the pyrosequenced regions in EN1, LHX2, PITX2 and APC. With respect to EN1, methylation of its far-upstream region was positively associated with expression in a comparison of normal cell cultures. This evidence concerns the gene PITX2 and cancer.