Eight of the 23 examined DNA regions overlapped or were near regions previously reported to be hypermethylated in breast cancer vs. non-cancerous breast tissue, namely, EGFR [20], GSTP1 [21], LHX2 [22], PITX2 [23], RASSF1A [24], RUNX3 [25], APC [26] and BRCA1 [27, 28] or hypomethylated in breast cancer vs. normal breast, namely, TFF1 [29], satellite 2 and LINE-1, DNA repeats [30, 31]. The gene discussed is PITX2; the disease is breast cancer.