A cohort of 21 CRC patients, treated with a peptide-cocktail vaccine derived from RNF43 and translocase of the outer mitochondrial membrane 34 (TOMM34), showed antigen-specific CTL responses to both RNF43 and TOMM34 in 8/21 and CTL response to only one of the peptides in 12 out of 21 patients. This evidence concerns the gene RNF43 and colorectal carcinoma.