PIWIL1 and endometrial cancer: To determine whether Piwil1 could enhance the stemness of endometrial cancer cells by inducing EMT, we transfected Piwil1 expression plasmids into HEC-1B cell lines to originate Piwil1 overexpression cells (HEC-1BexPiwil1 cells) and transfected shRNA against Piwil1 to Ishikawa cell lines to originate Piwil1 knock-down cells (IshikawashPiwil1 cells).