Chi-square analysis revealed that the expression of WWP1 in HCC tissues was highly correlated with tumor size (P = 0.015), histological grade (P < 0.001), TNM stage (P < 0.001), vascular invasion (P = 0.018) and tumor capsule (P = 0.026) but not with age, sex, HBV, serum AFP or liver cirrhosis (Table 1). This evidence concerns the gene AFP and cirrhosis of liver.