To further investigate how ZFAS1 down-regulation induces CRC cells growth arrest and apoptosis, we examined the levels of p53, CDK1, CDK1 partner cyclin B1 and PARP cleavage for detection of apoptosis after silencing of ZFAS1 in HCT116+/+ and DLD-1 cells. This evidence concerns the gene TP53 and colorectal carcinoma.